Insemination of Eggs and Embryo Culture

During egg insemination for in vitro fertilization, Georgia Regents Reproductive Medicine and Infertility Associates in Augusta, Georgia may use intracytoplasmic sperm injection (ICSI) or donors, especially in cases of severe male factor.

Following egg retrieval, the follicular fluid is immediately transferred to the adjacent laboratory for identification of eggs, evaluation, and preparation for insemination. In the process of collecting the follicular fluid, it is typical for a large number of eggs to be retrieved.

Intracytoplasmic Sperm Injection (ICSI)

The prepared sperm will be added to each egg and they will be allowed to incubate overnight under controlled laboratory conditions. The next day, each egg is evaluated for evidence of fertilization.

However, it is possible that no eggs are fertilized. If this happens, the laboratory staff may re-inseminate the eggs or perform intracytoplasmic sperm injection (ICSI) in hopes of obtaining embryos for transfer.

If fertilization still does not occur, the eggs will be discarded and the remainder of the procedure will be cancelled.

Severe Male Factor

In the case of severe male factor, the couple may be asked to consider the option of using anonymous donor sperm (obtained through a licensed sperm bank for use as a "backup" or secondary sperm source) if it is not possible to obtain sufficient sperm from the partner at the time of fertilization.

The eggs that have fertilized will be allowed to develop for one or more additional days under controlled laboratory conditions before they are placed inside the woman's uterus.

Depending upon the couple's wishes, some fertilized eggs or embryos may be frozen and stored for future use.

After the embryos are transferred to the womb, the woman will continue progesterone supplementation that begins on the evening of the egg retrieval procedure. Progesterone is usually taken by injection. Administration of this medication after egg collection has been shown to create a more favorable uterine environment for the embryos, which therefore increases pregnancy rates.

Please contact us with questions about in vitro fertilization (IVF), or if you want to schedule an appointment for an in vitro fertilization and embryo transfer.